Which cells are in midbrain organoids?
Neurons
The main transmitters of information in the nervous system. We can identify them with a TUJ1 marker (red).
Human midbrain organoids
Astrocytes
Astrocytes are star-shaped cells support others - such as neurons - in the brain. We can identify them with a GFAP marker (green).
Midbrain organoids are 3D in vitro models that recapitulate the cellular features of the human midbrain. They can be generated from somatic samples taken from any healthy individual or PD patient.
Dopaminergic neurons
This subtype of neurons degenerates in Parkinson’s Disease, and they are especially abundant in a region called the midbrain. We can identify them with a TH marker (white).
Microglia (green) identified by IBA1 marker expression in organoids (left) and 2D (right).
… But we can also find many other cell types in midbrain organoids; such as progenitor cells, stem cells, other neuronal subtypes and microglia.
